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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA000233 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA000232 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in bone marrow poietic cells.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
Immunohistochemical staining of human bone marrow shows nuclear positivity in hematopoietic cells.
Expression
RNA: detected in 4 tissues Protein: detected in 12 cell types
RNA: detected in 4 tissues Protein: detected in 12 cell types
RNA: detected in 4 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH9
HIER pH6
Antibody dilution
1:15
1:25
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000232
Antibody HPA000233
Antibody CAB009195
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400758)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400758)
Target mass (kDa)
42.8, 35.3
42.8
42.8, 35.3
Antibody dilution
1:125
1:125
1:500
PROTEIN ARRAY
Antibody HPA000232
Antibody HPA000233
Antibody CAB009195
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
