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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:5
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA003103
Antibody HPA037536
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity of basal cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in basal cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 21 cell types
RNA: detected in 37 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:300
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Approved
Figure description
Predominant distribution in blood vessels throughout the forebrain and additional staining in neuronal cells from the forebrain (in cortical and septal areas among others) and few other areas of the hindbrain.
WESTERN BLOT
Antibody HPA003103
Antibody HPA037536
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
109.8, 105
109.8, 105
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA003103
Antibody HPA037536
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
