Expression of MSLN in cancer - Summary - The Human Protein Atlas

GENERAL INFORMATION
Gene name	MSLN
Gene description	Mesothelin
Protein class	Cancer-related genes Disease related genes Plasma proteins Predicted intracellular proteins Predicted membrane proteins Predicted secreted proteins
Predicted localization	Intracellular,Membrane,Secreted
Number of transcripts	9
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Tissue enhanced (ovarian cancer)
HPA (cell line):	Cell line enhanced (CAPAN-2, EFO-21, HeLa, hTCEpi)
HPA (normal tissue):	Group enriched (fallopian tube, lung)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in squamous epithelia and glandular cells of cervix and fallopian tube.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data.
Reliability score	Supported based on 2 antibodies. HPA017172 , CAB002216
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PROGNOSTIC SUMMARY
Prognostic marker in renal cancer (favourable) and pancreatic cancer (unfavourable).
Renal cancer p<0.001 Pancreatic cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Tissue enhanced (ovarian cancer)

PROTEIN EXPRESSION

STAINING SUMMARY

HPA017172	CAB002216

GENE INFORMATION
Gene name	MSLN (HGNC Symbol)
Synonyms	CAK1, MPF
Description	Mesothelin (HGNC Symbol)
Entrez gene summary	This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
Chromosome	16
Cytoband	p13.3
Chromosome location (bp)	643262 - 768865
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000102854 (version 83.38)
Entrez gene	10232
UniProt	Q13421 (UniProt - Evidence at protein level)
neXtProt	NX_Q13421
Antibodypedia	MSLN antibodies

PROTEIN BROWSER

MSLN-001	MSLN-002	MSLN-003	MSLN-004	MSLN-005	MSLN-006	MSLN-007	MSLN-008	MSLN-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
MSLN-001 ENSP00000372313 ENST00000382862	Q13421 [Direct mapping] Mesothelin Megakaryocyte-potentiating factor Mesothelin, cleaved form Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Cancer-related genes Candidate cancer biomarkers Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005615 [extracellular space] GO:0005794 [Golgi apparatus] GO:0005886 [plasma membrane] GO:0007155 [cell adhesion] GO:0007160 [cell-matrix adhesion] GO:0009986 [cell surface] GO:0016020 [membrane] GO:0031016 [pancreas development] GO:0031225 [anchored component of membrane] Show all	630 aa 69 kDa	Yes	1
MSLN-002 ENSP00000456702 ENST00000566549	Q13421 [Direct mapping] Mesothelin Megakaryocyte-potentiating factor Mesothelin, cleaved form Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Cancer-related genes Candidate cancer biomarkers Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005794 [Golgi apparatus] GO:0005886 [plasma membrane] GO:0007155 [cell adhesion] GO:0007160 [cell-matrix adhesion] GO:0009986 [cell surface] GO:0016020 [membrane] GO:0031225 [anchored component of membrane] Show all	622 aa 68.1 kDa	Yes	1
MSLN-003 ENSP00000456008 ENST00000563941	Q13421 [Direct mapping] Mesothelin Megakaryocyte-potentiating factor Mesothelin, cleaved form Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Cancer-related genes Candidate cancer biomarkers Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005794 [Golgi apparatus] GO:0005886 [plasma membrane] GO:0007155 [cell adhesion] GO:0007160 [cell-matrix adhesion] GO:0009986 [cell surface] GO:0016020 [membrane] GO:0031225 [anchored component of membrane] Show all	622 aa 68.1 kDa	Yes	1
MSLN-004 ENSP00000442965 ENST00000545450	Q13421 [Direct mapping] Mesothelin Megakaryocyte-potentiating factor Mesothelin, cleaved form Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Cancer-related genes Candidate cancer biomarkers Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005794 [Golgi apparatus] GO:0005886 [plasma membrane] GO:0007155 [cell adhesion] GO:0007160 [cell-matrix adhesion] GO:0009986 [cell surface] GO:0016020 [membrane] GO:0031225 [anchored component of membrane] Show all	622 aa 68.1 kDa	Yes	1
MSLN-005 ENSP00000454295 ENST00000566269	H3BMA1 [Direct mapping] Mesothelin Show all	MEMSAT3 predicted membrane proteins SCAMPI predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		334 aa 37.6 kDa	No	0
MSLN-006 ENSP00000457847 ENST00000561896	H3BUX1 [Direct mapping] Mesothelin Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		398 aa 43.8 kDa	No	0
MSLN-007 ENSP00000458003 ENST00000569566	H3BV92 [Direct mapping] Mesothelin Show all	MEMSAT3 predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		170 aa 18.2 kDa	Yes	0
MSLN-008 ENSP00000456132 ENST00000563651	H3BR90 [Direct mapping] Mesothelin Show all	MEMSAT3 predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		333 aa 36.8 kDa	Yes	0
MSLN-201 ENSP00000482893 ENST00000620831	Q13421 [Direct mapping] Mesothelin Megakaryocyte-potentiating factor Mesothelin, cleaved form Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Cancer-related genes Candidate cancer biomarkers Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005794 [Golgi apparatus] GO:0005886 [plasma membrane] GO:0007155 [cell adhesion] GO:0007160 [cell-matrix adhesion] GO:0009986 [cell surface] GO:0016020 [membrane] GO:0031225 [anchored component of membrane] Show all	630 aa 69 kDa	Yes	1