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GDPD3

ANTIBODY INFORMATION
 
 

Antibody HPA041148

  

Antibody HPA041470

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041148   HPA041470  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

   1  

Proper citation

 Atlas Antibodies Cat#HPA041148, RRID:AB_10794545   Atlas Antibodies Cat#HPA041470, RRID:AB_10795121  

VALIDATION SUMMARY
 
 

Antibody HPA041148

  

Antibody HPA041470

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041148

  

Antibody HPA041470

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytokinetic bridge.  

Antibody dilution

   1:44  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041148

  

Antibody HPA041470

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and nuclear positivity in urothelial cells.   Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in umbrella cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 61 cell types		   RNA: detected in 34 tissues
Protein: detected in 39 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:225   1:35  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041148

  

Antibody HPA041470

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 36.6   36.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041148

  

Antibody HPA041470

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041148

  

Antibody HPA041470

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   62  

Antigen sequence

 
DLPLYKEKLEVYFSPGHFAHGSDRRMVRLEDLFQRFPRTPMSVEIKGKNE
ELIREIAGLVRRYDRNEITIW
  
LRRPHLLHTPRAPTFRIRLGAHRGGSGELLENTMEAMENSMAQRSDLLEL
DCQLTRDRVVVV
 

Matching transcripts

 GDPD3-001 - ENSP00000384363 [100%]
  GDPD3-001 - ENSP00000384363 [100%]
 

Other gene match

    
ANTIGEN VIEW
GDPD3-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA041148

  

Antibody HPA041470

  

1.

   Identification and Validation of Protein Biomarkers of Response to Neoadjuvant Platinum Chemotherapy in Muscle Invasive Urothelial Carcinoma
Baras AS et al
PLoS One 2015;10(7):e0131245
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.