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Antibody staining overlaps with antibody HPA043586.
Validated
Antibody staining overlaps with antibody HPA043019.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:85
1:23
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA043019
Antibody HPA043586
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 65 cell types
RNA: detected in 37 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:35
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043019
Antibody HPA043586
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420027)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
97.2, 88.7, 86
88.7, 86
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA043019
Antibody HPA043586
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
