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NOMO1

ANTIBODY INFORMATION
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antibody CAB025192

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA046697   HPA061174   AF3755  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

 NOMO3 - ENSG00000103226 [100%]
NOMO2 - ENSG00000185164 [100%]
   NOMO3 - ENSG00000103226 [100%]
NOMO2 - ENSG00000185164 [100%]
    

Released in version

 13   13   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA046697, RRID:AB_2679758   Atlas Antibodies Cat#HPA061174, RRID:AB_2684444    

VALIDATION SUMMARY
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antibody CAB025192

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Uncertain   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antibody CAB025192

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.  

Antibody dilution

     1:29  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antibody CAB025192

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 22 tissues
Protein: detected in 68 cell types		   RNA: detected in 22 tissues
Protein: detected in 63 cell types		   RNA: detected in 22 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:160   1:200   1:115  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antibody CAB025192

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 139.5, 139.4, 134.3, 134.2, 134.1, 122.1   139.5, 139.4, 134.3, 134.2, 134.1, 122.1, 31.5   139.5, 134.3, 122.1, 16  

Antibody dilution

 1:260   1:170   1:500  

PROTEIN ARRAY
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antibody CAB025192

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2050   1:2050    

ANTIGEN INFORMATION
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antibody CAB025192

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 94   86    

Antigen sequence

 
GEKITVTPSSKELLFYPPSMEAVVSGESCPGKLIEIHGKAGLFLEGQIHP
ELEGVEIVISEKGASSPLITVFTDDKGAYSVGPLHSDLEYTVTS
  
FRFEPSSQMIEVQEGQNLKITITGYRTAYSCYGTVSSLNGEPEQGVAMEA
VGQNDCSIYGEDTVTDEEGKFRLRGLLPGCVYHVQL
  

											 
																				

																		

										
											
Matching transcripts

												
																					NOMO1-001 - ENSP00000287667 [100%] 
NOMO1-201 - ENSP00000479933 [100%] 
NOMO1-202 - ENSP00000483827 [100%] 

											 
																						NOMO1-001 - ENSP00000287667 [100%] 
NOMO1-201 - ENSP00000479933 [100%] 
NOMO1-202 - ENSP00000483827 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					NOMO3 - ENSG00000103226 [100%]
NOMO2 - ENSG00000185164 [100%]

											 
																						NOMO3 - ENSG00000103226 [100%]
NOMO2 - ENSG00000185164 [100%]

											 
																						
											 
																				

																		

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