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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:11
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA009285
Antibody HPA017139
Antibody CAB017826
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human prostate shows strong membrane and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 65 cell types
RNA: detected in 36 tissues Protein: detected in 69 cell types
RNA: detected in 36 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:15
1:25
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA009285
Antibody HPA017139
Antibody CAB017826
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410803)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410803)
