Fields »
ABOUT
HELP
BLOG
JPH1

ANTIBODY INFORMATION
 
 

Antibody HPA008996

  

Antibody HPA009413

  

Antibody CAB013461

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
  

Product name

 HPA008996   HPA009413   40-5200  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 4   3   3  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA008996, RRID:AB_1852023   Atlas Antibodies Cat#HPA009413, RRID:AB_1079163    

VALIDATION SUMMARY
 
 

Antibody HPA008996

  

Antibody HPA009413

  

Antibody CAB013461

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008996

  

Antibody HPA009413

  

Antibody CAB013461

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA009413.		  
Validated

Antibody staining overlaps with antibody HPA008996.		    
 

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus.    

Antibody dilution

 1:40   1:25    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008996

  

Antibody HPA009413

  

Antibody CAB013461

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 20 tissues
Protein: detected in 38 cell types		   RNA: detected in 20 tissues
Protein: detected in 31 cell types		   RNA: detected in 20 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:35   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008996

  

Antibody HPA009413

  

Antibody CAB013461

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 71.7   71.7   71.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008996

  

Antibody HPA009413

  

Antibody CAB013461

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA008996

  

Antibody HPA009413

  

Antibody CAB013461

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 126   102    

Antigen sequence

 
KADAADQAALAARQECDIARAVARELSPDFYQPGPDYVKQRFQEGVDAKE
NPEEKVPEKPPTPKESPHFYRKGTTPPRSPEASPKHSHSPASSPKPLKKQ
NPSSGARLNQDKRSVADEQVTAIVNK
  
PQSKYSGRHHIPNPSNGELHSQYHGYYVKLNAPQHPPVDVEDGDGSSQSS
SALVHKPSANKWSPSKSVTKPVAKESKAEPKAKKSELAIPKNPASNDSCP
AL
  

											 
																				

																		

										
											
Matching transcripts

												
																					JPH1-001 - ENSP00000344488 [100%] 

											 
																						JPH1-001 - ENSP00000344488 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
JPH1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008996

												
										 
																				
											
											
											
Antibody HPA009413

												
										 
																				
											
											
											
Antibody CAB013461

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Triadin/Junctin double null mouse reveals a differential role for Triadin and Junctin in anchoring CASQ to the jSR and regulating Ca(2+) homeostasis

	Boncompagni S et al

	PLoS One 2012;7(7):e39962

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.