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Antibody staining overlaps with antibody HPA061291.
Validated
Antibody staining overlaps with antibody HPA059928.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & nucleoli.
Antibody dilution
1:10
1:71
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA059928
Antibody HPA061291
Standard validation
Approved
Figure description
Immunohistochemical staining of human tonsil shows strong nuclear positivity in,non-germinal center cells, germinal center cells were moderately stained. .
Expression
RNA: detected in 37 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA059928
Antibody HPA061291
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
10.6
10.6, 8.9, 5.8
Antibody dilution
1:80
1:240
PROTEIN ARRAY
Antibody HPA059928
Antibody HPA061291
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.