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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.
Antibody dilution
1:26
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043536
Standard validation
Approved
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
Antibody dilution
1:120
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043536
Standard validation
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402526)
Target mass (kDa)
55.2, 54.8, 44.9
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA043536
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
