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Immunohistochemical staining of human retina shows strong nuclear positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong nuclear positivity in photoreceptor cells.
Expression
RNA: detected in 0 tissues Protein: detected in 0 cell types
RNA: detected in 0 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1200
1:100
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA036762
Antibody HPA036763
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424645)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424645)
Target mass (kDa)
32.3
32.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA036762
Antibody HPA036763
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA036762
Antibody HPA036763
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
50
Antigen sequence
PLPEAQRAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYGSPSSYFSGLD
PYLSPMVPQLGGPALSPLSGPSVGPSLAQSPTSLSGQSYGAYSPVDSLEF
K
