ISYNA1 - Antibodies - The Human Protein Atlas

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ISYNA1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA007931

Antibody HPA008232

Antibody CAB020720

Provider

Atlas Antibodies
Sigma-Aldrich

Origene

Product name

HPA007931

HPA008232

3035.00.02

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA007931, RRID:AB_2667940

Atlas Antibodies Cat#HPA008232, RRID:AB_2668018

VALIDATION SUMMARY

Antibody HPA007931

Antibody HPA008232

Antibody CAB020720

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Uncertain

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA007931

Antibody HPA008232

Antibody CAB020720

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & cytosol.

Antibody dilution

1:50

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA007931

Antibody HPA008232

Antibody CAB020720

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA008232 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA007931 across 45 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA008232 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA007931 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.

Immunohistochemical staining of human testis shows cytoplasmic positivity of cells in seminiferous ducts.

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Expression

RNA: detected in 36 tissues
Protein: detected in 33 cell types

RNA: detected in 36 tissues
Protein: detected in 18 cell types

RNA: detected in 36 tissues
Protein: detected in 47 cell types

Retrieval

HIER pH6

Antibody dilution

1:1800

1:1000

1:5200

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA007931

Antibody HPA008232

Antibody CAB020720

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

61.1, 55.1, 47.1, 25.2

61.1, 55.1, 47.1

61.1, 55.1, 47.1, 25.2, 19.5, 16.1

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA007931

Antibody HPA008232

Antibody CAB020720

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA007931

Antibody HPA008232

Antibody CAB020720

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

119

109

Antigen sequence

SVLVDFLIGSGLKTMSIVSYNHLGNNDGENLSAPLQFRSKEVSKSNVVDD
MVQSNPVLYTPGEEPDHCVVIKYVPYVGDSKRALDEYTSELMLGGTNTLV
LHNTCEDSLLAAPIMLDLA

VYIPEFIAANQSARADNLIPGSRAQQLEQIRRDIRDFRSSAGLDKVIVLW
TANTERFCEVIPGLNDTAENLLRTIELGLEVSPSTLFAVASILEGCAFLN
GSPQNTLVP

Matching transcripts

ISYNA1-001 - ENSP00000415458 [100%]
ISYNA1-002 - ENSP00000337746 [100%]
ISYNA1-006 - ENSP00000475677 [100%]
ISYNA1-011 - ENSP00000464528 [83%]

ISYNA1-001 - ENSP00000415458 [100%]
ISYNA1-002 - ENSP00000337746 [100%]
ISYNA1-006 - ENSP00000475677 [100%]

Other gene match

ANTIGEN VIEW

ISYNA1-001	ISYNA1-002	ISYNA1-006	ISYNA1-011

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.