We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & nuclear membrane.
Antibody dilution
1:54
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA042072
Antibody HPA045480
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.
Immunohistochemical staining of human kidney shows moderate nuclear and cytoplasmic positivity in renal tubules.
Expression
RNA: detected in 36 tissues Protein: detected in 62 cell types
RNA: detected in 36 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:400
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042072
Antibody HPA045480
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
194.6
194.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA042072
Antibody HPA045480
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
