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ANTIBODY INFORMATION
 
 

Antibody HPA047776

  

Antibody HPA057722

  

Antibody CAB034349

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA047776   HPA057722   sc-130058  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   13   7  

References

      

Proper citation

 Atlas Antibodies Cat#HPA047776, RRID:AB_2680151     Santa Cruz Biotechnology Cat#sc-130058, RRID:AB_2251220  

VALIDATION SUMMARY
 
 

Antibody HPA047776

  

Antibody HPA057722

  

Antibody CAB034349

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Approved     Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047776

  

Antibody HPA057722

  

Antibody CAB034349

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles & cell junctions.    

Antibody dilution

   1:37    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047776

  

Antibody HPA057722

  

Antibody CAB034349

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.     Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells of white and red pulp.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 43 cell types		     RNA: detected in 37 tissues
Protein: detected in 38 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:300     1:225  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047776

  

Antibody HPA057722

  

Antibody CAB034349

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 55.5, 41.9   55.5   55.5, 41.9, 10.5, 7.2  

Antibody dilution

 1:170   1:120   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047776

  

Antibody HPA057722

  

Antibody CAB034349

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1350   1:1350    

ANTIGEN INFORMATION
 
 

Antibody HPA047776

  

Antibody HPA057722

  

Antibody CAB034349

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 94   87    

Antigen sequence

 
QETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTI
TAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGED
  
GSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRL
SLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKV
  

											 
																				

																		

										
											
Matching transcripts

												
																					NAMPT-001 - ENSP00000222553 [100%] 
NAMPT-002 - ENSP00000346242 [100%] 

											 
																						NAMPT-001 - ENSP00000222553 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NAMPT-001
NAMPT-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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