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Cytoplasmic region of segmented cells in 10x-images
Antibody dilution
1:72
Standard validation
Approved
Figure description
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Antibody dilution
1:72
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA050294
Antibody HPA060836
Antibody CAB021103
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA060836 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA050294 across 72 cell types.
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human tonsil shows cytoplasmic positivity mainly in germinal center cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells and moderate cytoplasmic positivity in exocrine pancreas.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 60 cell types
RNA: detected in 37 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:400
1:350
1:500
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050294
Antibody HPA060836
Antibody CAB021103
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Target mass (kDa)
20.6
20.6
20.6, 4.4
Antibody dilution
1:360
1:170
1:1000
PROTEIN ARRAY
Antibody HPA050294
Antibody HPA060836
Antibody CAB021103
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
