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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human breast shows distinct nuclear positivity in a subset of glandular cells.
Expression
RNA: detected in 24 tissues Protein: detected in 66 cell types
RNA: detected in 24 tissues Protein: detected in 78 cell types
RNA: detected in 24 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:225
1:1100
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029730
Antibody HPA029731
Antibody CAB016217
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400759)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
48, 47.9
48, 47.9
48, 47.9
Antibody dilution
1:125
1:250
1:500
PROTEIN ARRAY
Antibody HPA029730
Antibody HPA029731
Antibody CAB016217
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
