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EDRF1

ANTIBODY INFORMATION
 
 

Antibody HPA031820

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031820  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 6  

References

  

Proper citation

  

VALIDATION SUMMARY
 
 

Antibody HPA031820

  
Immunocytochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031820

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.  

Antibody dilution

 1:83  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA031820

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 138.5, 134.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA031820

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000  

ANTIGEN INFORMATION
 
 

Antibody HPA031820

  

Antigen

 Recombinant protein fragment  

Length (aa)

 112  

Antigen sequence

 
SLRLRDNNKPINVLTGIDYWLDNLICNVPELVMCFHVNGIVQKYEMIKTE
EIPNLENSNFSTKVIKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKL
YDLTTLCEETED
 

Matching transcripts

 EDRF1-001 - ENSP00000336727 [100%]
EDRF1-007 - ENSP00000349244 [100%]
 

Other gene match

  
ANTIGEN VIEW
EDRF1-001
EDRF1-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.