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GOSR2

ANTIBODY INFORMATION
 
 

Antibody HPA048956

  

Antibody HPA054472

  

Antibody CAB017456

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Enzo Life Sciences
  

Product name

 HPA048956   HPA054472   ADI-VAP-PT049  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 14   11   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA048956, RRID:AB_2680569   Atlas Antibodies Cat#HPA054472, RRID:AB_2682499   Enzo Life Sciences Cat#ADI-VAP-PT049, RRID:AB_10532285  

VALIDATION SUMMARY
 
 

Antibody HPA048956

  

Antibody HPA054472

  

Antibody CAB017456

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

  Uncertain   Uncertain  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048956

  

Antibody HPA054472

  

Antibody CAB017456

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & the Golgi apparatus.    

Antibody dilution

 1:37   1:79    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048956

  

Antibody HPA054472

  

Antibody CAB017456

  

Standard validation

   Uncertain   Uncertain  

     

Figure description

   Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 47 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:550   1:150  

Literature conformity

   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048956

  

Antibody HPA054472

  

Antibody CAB017456

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 24.8, 24.6, 23.1, 22.7, 19.5, 17.8, 16.8   24.8, 24.6, 23.1   24.8, 24.6, 23.1, 22.7, 19.5, 17.8, 16.8, 11.1  

Antibody dilution

 1:190   1:400   1:500  

PROTEIN ARRAY
 
 

Antibody HPA048956

  

Antibody HPA054472

  

Antibody CAB017456

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:10000   1:3150    

ANTIGEN INFORMATION
 
 

Antibody HPA048956

  

Antibody HPA054472

  

Antibody CAB017456

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 96   85    

Antigen sequence

 
PLFQQTHKQVHEIQSCMGRLETADKQSVHIVENEIQASIDQIFSRLERLE
ILSSKEPPNKRQNARLRVDQLKYDVQHLQTALRNFQHRRHAREQQG
  
LLSRTFTTNDSDTTIPMDESLQFNSSLQKVHNGMDDLILDGHNILDGLRT
QRLTLKGTQKKILDIANMLGLSNTVMRLIEKRAFQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					GOSR2-001 - ENSP00000225567 [99%] 
GOSR2-002 - ENSP00000377101 [99%] 
GOSR2-003 - ENSP00000394559 [99%] 
GOSR2-004 - ENSP00000461673 [99%] 
GOSR2-006 - ENSP00000458911 [99%] 
GOSR2-009 - ENSP00000458154 [98%] 
GOSR2-008 - ENSP00000460760 [82%] 

											 
																						GOSR2-001 - ENSP00000225567 [100%] 
GOSR2-002 - ENSP00000377101 [100%] 
GOSR2-009 - ENSP00000458154 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GOSR2-001
GOSR2-002
GOSR2-003
GOSR2-004
GOSR2-006
GOSR2-008
GOSR2-009
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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