SLC6A4 - Antibodies - The Human Protein Atlas

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SLC6A4

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA054723

Antibody HPA074728

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA054723

HPA074728

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA074728, RRID:AB_2686708

VALIDATION SUMMARY

Antibody HPA054723

Antibody HPA074728

Immunocytochemistry

Approved

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA054723

Antibody HPA074728

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus & vesicles.

Antibody dilution

1:6

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA054723

Antibody HPA074728

Standard validation

Supported

Figure description

Labelling of many serotonergic fibres throughout the brain.

WESTERN BLOT

Antibody HPA054723

Antibody HPA074728

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

70.3

Antibody dilution

1:200

PROTEIN ARRAY

Antibody HPA054723

Antibody HPA074728

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1600

1:500

ANTIGEN INFORMATION

Antibody HPA054723

Antibody HPA074728

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

ITPGTFKERIIKSITPETPTEIPCGDIRLNAV

KNSWNTGNCTNYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGG
I

Matching transcripts

SLC6A4-002 - ENSP00000385822 [100%]
SLC6A4-201 - ENSP00000261707 [100%]

SLC6A4-001 - ENSP00000378298 [100%]
SLC6A4-002 - ENSP00000385822 [100%]
SLC6A4-201 - ENSP00000261707 [100%]

Other gene match

ANTIGEN VIEW

SLC6A4-001	SLC6A4-002	SLC6A4-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.