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HSD17B1

ANTIBODY INFORMATION
 
 

Antibody HPA021032

  

Antibody HPA065296

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA021032   HPA065296  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA021032, RRID:AB_1844345   Atlas Antibodies Cat#HPA065296, RRID:AB_2685457  

VALIDATION SUMMARY
 
 

Antibody HPA021032

  

Antibody HPA065296

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021032

  

Antibody HPA065296

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:20    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021032

  

Antibody HPA065296

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in Syncytiotrophoblasts.   Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in trophoblastic cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 5 cell types		   RNA: detected in 35 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021032

  

Antibody HPA065296

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 35, 34.9   35, 34.9  

Antibody dilution

 1:250   1:380  

PROTEIN ARRAY
 
 

Antibody HPA021032

  

Antibody HPA065296

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:4600  

ANTIGEN INFORMATION
 
 

Antibody HPA021032

  

Antibody HPA065296

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 76   35  

Antigen sequence

 
FGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQV
FREAAQNPEEVAEVFLTALRAPKPTL
  
ALDPSQSFKVYATLRDLKTQGRLWEAARALACPQG
 

Matching transcripts

 HSD17B1-001 - ENSP00000466799 [100%]
HSD17B1-002 - ENSP00000225929 [99%]
  HSD17B1-001 - ENSP00000466799 [94%]
HSD17B1-002 - ENSP00000225929 [94%]
 

Other gene match

    
ANTIGEN VIEW
HSD17B1-001
HSD17B1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.