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ANTIBODY INFORMATION
 
 

Antibody HPA027305

  

Antibody CAB004031

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
  

Product name

 HPA027305   3035  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 5   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA027305, RRID:AB_1854429   Cell Signaling Technology Cat#3035, RRID:AB_330564  

VALIDATION SUMMARY
 
 

Antibody HPA027305

  

Antibody CAB004031

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027305

  

Antibody CAB004031

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:100    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027305

  

Antibody CAB004031

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 40 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027305

  

Antibody CAB004031

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 105.4, 85.5   105.4, 85.5, 21.9, 21.4, 15.9, 8.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA027305

  

Antibody CAB004031

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA027305

  

Antibody CAB004031

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 143    

Antigen sequence

 
GTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHGTMDTESKKDPEG
CDKSDDKNTVNLFGKVIETTEQDQEPSEATVGNGEVTLTYATGTKEESAG
VQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAV
  

											 
																				

																		

										
											
Matching transcripts

												
																					NFKB1-001 - ENSP00000226574 [100%] 
NFKB1-002 - ENSP00000378297 [100%] 
NFKB1-003 - ENSP00000424790 [100%] 
NFKB1-013 - ENSP00000469340 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NFKB1-001
NFKB1-002
NFKB1-003
NFKB1-013
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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