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Cell line enhanced (BEWO, CACO-2, Hep G2, NB-4, RPMI-8226, SH-SY5Y)
HPA (normal tissue):
Group enriched (duodenum, small intestine)
Protein evidence
Evidence at protein level
Protein expression normal tissue
High expression in small intestine and distinct positivity in plasma.
ANTIBODY IHC RELIABILITY
Data reliability description
Antibody staining mainly consistent with RNA expression data. Secreted protein, tissue location of RNA and protein might differ and correlation is complex.
Fraction of cells in few cases of endometrial and liver cancers exhibited weak to moderate cytoplasmic positivity. Membranous positivity was noted in few cases of urothelial cancer. Remaining cancer tissues were mostly negative.
Most papillary adenocarcinomas of thyroid exhibited moderate cytoplasmic and/or nuclear staining. Few renal, stomach and pancreatic cancers were weak to moderately stained. Remaining cancer tissues were negative.
Several renal cell carcinomas and skin cancers along with few cases of pancreatic thyroid and stomach cancers exhibited weak to moderate cytoplasmic immunoreactivity. Other cancer tissues were in general negative.
Few cases of duct carcinomas of breast, melanoma, carcinoid, renal cancers along with head and neck cancers showed moderate cytoplasmic staining. A single case of embryonal carcinoma of testis and renal cancer displayed strong membranous and cytoplasmic positivity. A single case of papillary adenocarcinoma of thyroid showed strong cytoplasmic and nuclear immunoreactivity. Remaining cancer tissues were weakly stained or negative.
GENE INFORMATION
Gene name
APOA4
Synonyms
Description
Apolipoprotein A-IV (HGNC Symbol)
Entrez gene summary
Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]