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Immunohistochemical staining of human lymph node shows strong membranous positivity in non-germinal center cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp and cells in white pulp.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells outside the reaction centra.
Expression
RNA: detected in 27 tissues Protein: detected in 15 cell types
RNA: detected in 27 tissues Protein: detected in 11 cell types
RNA: detected in 27 tissues Protein: detected in 15 cell types
RNA: detected in 27 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:75
1:50
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043416
Antibody HPA060839
Antibody CAB015392
Antibody CAB020308
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.6
54.6
54.6, 18.4
54.6, 18.4
Antibody dilution
1:250
1:80
1:500
1:500
PROTEIN ARRAY
Antibody HPA043416
Antibody HPA060839
Antibody CAB015392
Antibody CAB020308
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
