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PRPF40B

ANTIBODY INFORMATION
 
 

Antibody HPA038426

  

Antibody HPA038599

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038426   HPA038599  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA038426, RRID:AB_10674684   Atlas Antibodies Cat#HPA038599, RRID:AB_10674689  

VALIDATION SUMMARY
 
 

Antibody HPA038426

  

Antibody HPA038599

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038426

  

Antibody HPA038599

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & cytosol.    

Antibody dilution

 1:111    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038426

  

Antibody HPA038599

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 59 cell types		   RNA: detected in 36 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:250  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038426

  

Antibody HPA038599

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 101.4, 99.4, 97.8   101.4, 99.4, 97.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038426

  

Antibody HPA038599

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA038426

  

Antibody HPA038599

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 59   89  

Antigen sequence

 
KSNSPESETDPEEKAGKESDEKEQEQDKDRELQQAELPNRSPGFGIKKEK
TGWDTSESE
  
PQPQPDPPPVPPGPTPVPTGLLEPEPGGSEDCDVLEATQPLEQGFLQQLE
EGPSSSGQHQPQQEEEESKPEPERSGLSWSNREKAKQAF
 

Matching transcripts

 PRPF40B-001 - ENSP00000369634 [100%]
PRPF40B-002 - ENSP00000261897 [100%]
PRPF40B-019 - ENSP00000448073 [100%]
  PRPF40B-001 - ENSP00000369634 [100%]
PRPF40B-002 - ENSP00000261897 [100%]
PRPF40B-019 - ENSP00000448073 [100%]
 

Other gene match

    
ANTIGEN VIEW
PRPF40B-001
PRPF40B-002
PRPF40B-019
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.