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CPSF6

ANTIBODY INFORMATION
 
 

Antibody HPA039973

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039973  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 8  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA039973, RRID:AB_10795242  

VALIDATION SUMMARY
 
 

Antibody HPA039973

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Supported  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039973

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:74  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.  

Antibody dilution

 1:74  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039973

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 80 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:450  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039973

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.5, 59.2, 52.3  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA039973

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA039973

  

Antigen

 Recombinant protein fragment  

Length (aa)

 71  

Antigen sequence

 
ISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIG
NLTWWTTDEDLTEAVHSLGVN
 

Matching transcripts

 CPSF6-001 - ENSP00000391774 [100%]
CPSF6-003 - ENSP00000391437 [100%]
CPSF6-004 - ENSP00000266679 [100%]
 

Other gene match

  
ANTIGEN VIEW
CPSF6-001
CPSF6-003
CPSF6-004

RELEVANT PUBLICATIONS
 
 

Antibody HPA039973

  

1.

 Quantitative microscopy of functional HIV post-entry complexes reveals association of replication with the viral capsid
Peng K et al
Elife 2014;3:e04114
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.