Fields »
ABOUT
HELP
BLOG
BACH2

ANTIBODY INFORMATION
 
 

Antibody HPA051384

  

Antibody HPA058384

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051384   HPA058384  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA051384, RRID:AB_2681462   Atlas Antibodies Cat#HPA058384, RRID:AB_2683700  

VALIDATION SUMMARY
 
 

Antibody HPA051384

  

Antibody HPA058384

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051384

  

Antibody HPA058384

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.  

Antibody dilution

   1:8  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051384

  

Antibody HPA058384

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human tonsil shows strong nuclear positivity in in germinal and non-germinal center cells.    

Expression

 RNA: detected in 22 tissues
Protein: detected in 45 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:400    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA051384

  

Antibody HPA058384

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 92.5   92.5  

Antibody dilution

 1:790   1:80  

PROTEIN ARRAY
 
 

Antibody HPA051384

  

Antibody HPA058384

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6350   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA051384

  

Antibody HPA058384

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 95   87  

Antigen sequence

 
ETMDSETAKMACPRDQMLPEPISFEAAAIPVAEKEEALLPEPDVPTDTKE
SSEKDALTQYPRYKKYQLACTKNVYNASSHSTSGFASTFREDNSS
  
SNSLKPGLARGQIKSEPPSEENEEESITLCLSGDEPDAKDRAGDVEMDRK
QPSPAPTPTAPAGAACLERSRSVASPSCLRSLFSITK
 

Matching transcripts

 BACH2-003 - ENSP00000257749 [100%]
BACH2-005 - ENSP00000345642 [100%]
BACH2-201 - ENSP00000437473 [100%]
  BACH2-003 - ENSP00000257749 [100%]
BACH2-005 - ENSP00000345642 [100%]
BACH2-201 - ENSP00000437473 [100%]
 

Other gene match

    
ANTIGEN VIEW
BACH2-003
BACH2-005
BACH2-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.