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E2F3

ANTIBODY INFORMATION
 
 

Antibody HPA065012

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA065012  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 14  

References

  

Proper citation

 Atlas Antibodies Cat#HPA065012, RRID:AB_2685403  

VALIDATION SUMMARY
 
 

Antibody HPA065012

  
Immunocytochemistry

Supported  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA065012

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.  

Antibody dilution

 1:128  

Literature conformity

 The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA065012

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 49.2, 37  

Antibody dilution

 1:430  

PROTEIN ARRAY
 
 

Antibody HPA065012

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:5150  

ANTIGEN INFORMATION
 
 

Antibody HPA065012

  

Antigen

 Recombinant protein fragment  

Length (aa)

 74  

Antigen sequence

 
TNNQDHNGNIPKPASKDLASTNSGHSDCSVSMGNLSPLASPANLLQQTED
QIPSNLEGPFVNLLPPLLQEDYLL
 

Matching transcripts

 E2F3-001 - ENSP00000262904 [100%]
E2F3-201 - ENSP00000443418 [100%]
 

Other gene match

  
ANTIGEN VIEW
E2F3-001
E2F3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.