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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & nuclear bodies.
Antibody dilution
1:91
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035648
Antibody HPA044555
Standard validation
Approved
Figure description
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia of glandular cells.
Expression
RNA: detected in 25 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035648
Antibody HPA044555
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418593)
Target mass (kDa)
24.2
24.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA035648
Antibody HPA044555
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
