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Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 28 tissues Protein: detected in 40 cell types
RNA: detected in 28 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:250
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007172
Antibody CAB026471
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412947)
Target mass (kDa)
86.3
86.3, 14.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA007172
Antibody CAB026471
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.