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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:10
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA003265
Antibody CAB017519
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in tubular cells and glomeruli.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in subsets of glomerular cells.
Expression
RNA: detected in 32 tissues Protein: detected in 60 cell types
RNA: detected in 32 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003265
Antibody CAB017519
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
17.5, 10.4, 6.7, 6.5
17.5, 10.4, 6.7, 6.5, 4.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003265
Antibody CAB017519
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
