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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:100
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028499
Antibody CAB003842
Antibody CAB018144
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.
Immunohistochemical staining of human placenta shows distinct cytoplasmic and membranous positivity in decidual cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.
Expression
RNA: detected in 36 tissues Protein: detected in 42 cell types
RNA: detected in 36 tissues Protein: detected in 19 cell types
RNA: detected in 36 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:100
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028499
Antibody CAB003842
Antibody CAB018144
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400921)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
124, 19.1, 16.6
124, 19.1, 16.6
124, 19.1, 16.6
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA028499
Antibody CAB003842
Antibody CAB018144
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
