HGD - Antibodies - The Human Protein Atlas

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HGD

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA047374

Antibody HPA052359

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA047374

HPA052359

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA047374, RRID:AB_2680019

Atlas Antibodies Cat#HPA052359, RRID:AB_2681798

VALIDATION SUMMARY

Antibody HPA047374

Antibody HPA052359

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Protein array

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA047374

Antibody HPA052359

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.

Antibody dilution

1:25

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA047374

Antibody HPA052359

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA052359 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA047374 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA052359 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA047374 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 20 tissues
Protein: detected in 16 cell types

RNA: detected in 20 tissues
Protein: detected in 17 cell types

Retrieval

HIER pH6

Antibody dilution

1:60

1:400

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA047374

Antibody HPA052359

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

50, 16.2

Antibody dilution

1:120

1:160

PROTEIN ARRAY

Antibody HPA047374

Antibody HPA052359

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

1:1250

ANTIGEN INFORMATION

Antibody HPA047374

Antibody HPA052359

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

QVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINS
VAFDHADPSIFTVLTAKSVRPGVAIADFVIFPP

AELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTC
PRSTNKRSWLYRILPSVSHKPFESIDEGQVTHNWDEVDPDPNQ

Matching transcripts

HGD-001 - ENSP00000283871 [100%]
HGD-006 - ENSP00000419163 [100%]

HGD-001 - ENSP00000283871 [100%]

Other gene match

ANTIGEN VIEW

HGD-001	HGD-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.