OGG1 - Antibodies - The Human Protein Atlas

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OGG1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA027514

Antibody CAB047301

Provider

Atlas Antibodies
Sigma-Aldrich

NCI-CPTC

Product name

HPA027514

CPTC-OGG1-1

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

VALIDATION SUMMARY

Antibody HPA027514

Antibody CAB047301

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027514

Antibody CAB047301

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Antibody dilution

1:10

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027514

Antibody CAB047301

Standard validation

Approved

Figure description

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 50 cell types

RNA: detected in 37 tissues
Protein: detected in 64 cell types

Retrieval

HIER pH6

Antibody dilution

1:10

1:50000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027514

Antibody CAB047301

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

47.2, 45.8, 40.1, 39.7, 38.8, 36.4, 36.1, 29, 22.2, 14.3

47.2, 45.8, 40.1, 39.7, 38.8, 36.4, 36.1, 29, 24.4, 22.2, 14.6, 14.3

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA027514

Antibody CAB047301

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA027514

Antibody CAB047301

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

DVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNI
ARITGMVERLCQAFGPRLIQL

Matching transcripts

OGG1-001 - ENSP00000306561 [100%]
OGG1-002 - ENSP00000342851 [100%]
OGG1-003 - ENSP00000305584 [100%]
OGG1-004 - ENSP00000344899 [100%]
OGG1-005 - ENSP00000373337 [100%]
OGG1-006 - ENSP00000305527 [100%]
OGG1-007 - ENSP00000345520 [100%]
OGG1-008 - ENSP00000403598 [100%]
OGG1-016 - ENSP00000399810 [100%]
OGG1-201 - ENSP00000303132 [100%]

Other gene match

ANTIGEN VIEW

OGG1-001	OGG1-002	OGG1-003	OGG1-004	OGG1-005	OGG1-006	OGG1-007	OGG1-008	OGG1-016	OGG1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.