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Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.
Antibody dilution
1:47
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA046091
Antibody HPA055273
Standard validation
Supported
Figure description
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046091
Antibody HPA055273
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415515)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
18.3, 11.8, 9.3
18.3
Antibody dilution
1:250
1:230
PROTEIN ARRAY
Antibody HPA046091
Antibody HPA055273
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.