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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Antibody dilution
1:90
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA034828
Antibody HPA049229
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 43 cell types
RNA: detected in 37 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:35
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034828
Antibody HPA049229
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403057)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403057)
Target mass (kDa)
58.9, 28.2
58.9, 28.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA034828
Antibody HPA049229
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
