We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.
Antibody dilution
1:49
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA007406
Antibody HPA007917
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human placenta shows membranous and cytoplasmic positivity in trophoblastic cells and stromal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 23 tissues Protein: detected in 66 cell types
RNA: detected in 23 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:15
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007406
Antibody HPA007917
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401271)
Target mass (kDa)
63.4
63.4, 37, 35.6
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA007406
Antibody HPA007917
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
