Fields »
ABOUT
HELP
BLOG
MSH6

ANTIBODY INFORMATION
 
 

Antibody HPA028376

  

Antibody HPA028446

  

Antibody CAB009091

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA028376   HPA028446   sc-10798  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 6   6   3  

References

      

Proper citation

 Atlas Antibodies Cat#HPA028376, RRID:AB_10598909   Atlas Antibodies Cat#HPA028446, RRID:AB_10602182   Santa Cruz Biotechnology Cat#sc-10798, RRID:AB_670083  

VALIDATION SUMMARY
 
 

Antibody HPA028376

  

Antibody HPA028446

  

Antibody CAB009091

  
Immunocytochemistry

Supported      
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028376

  

Antibody HPA028446

  

Antibody CAB009091

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.      

Antibody dilution

 1:200      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028376

  

Antibody HPA028446

  

Antibody CAB009091

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.   Immunohistochemical staining of human lymph node shows moderate nuclear positivity in reaction center cells.   Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 46 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:550   1:25  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028376

  

Antibody HPA028446

  

Antibody CAB009091

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 152.8, 138, 119.8, 36.6   152.8, 138, 121.1, 119.8   152.8, 138, 121.1, 119.8, 36.6, 17.6, 13.5, 11.7, 6.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA028376

  

Antibody HPA028446

  

Antibody CAB009091

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA028376

  

Antibody HPA028446

  

Antibody CAB009091

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 76   83    

Antigen sequence

 
MVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRK
AREFEKMNQSLRLFREVCLASERSTV
  
HSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDR
VVRREICRIITKGTQTYSVLEGDPSENYSKYLL
  

											 
																				

																		

										
											
Matching transcripts

												
																					MSH6-001 - ENSP00000234420 [100%] 
MSH6-201 - ENSP00000438580 [100%] 
MSH6-202 - ENSP00000446475 [100%] 
MSH6-203 - ENSP00000477844 [100%] 
MSH6-205 - ENSP00000482078 [100%] 

											 
																						MSH6-001 - ENSP00000234420 [100%] 
MSH6-201 - ENSP00000438580 [100%] 
MSH6-202 - ENSP00000446475 [100%] 
MSH6-203 - ENSP00000477844 [100%] 
MSH6-204 - ENSP00000480261 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MSH6-001
MSH6-201
MSH6-202
MSH6-203
MSH6-204
MSH6-205
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.