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Antibody staining overlaps with antibody HPA028277.
Validated
Antibody staining overlaps with antibody HPA026512.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Antibody dilution
1:7
1:7
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA026512
Antibody HPA028277
Standard validation
Approved
Figure description
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 74 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA026512
Antibody HPA028277
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402042)
Target mass (kDa)
40.7, 34.9
40.7, 34.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA026512
Antibody HPA028277
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
