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ABCD3

ANTIBODY INFORMATION
 
 

Antibody HPA032026

  

Antibody HPA032027

  

Antibody CAB032516

  

Antibody CAB075749

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA032026   HPA032027   sc-20973   AMAb90995  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 12   6   13   14  

References

   1      

Proper citation

 Atlas Antibodies Cat#HPA032026, RRID:AB_2674117   Atlas Antibodies Cat#HPA032027, RRID:AB_10602103   Santa Cruz Biotechnology Cat#sc-20973, RRID:AB_2257637   Atlas Antibodies Cat#AMAb90995, RRID:AB_2665755  

VALIDATION SUMMARY
 
 

Antibody HPA032026

  

Antibody HPA032027

  

Antibody CAB032516

  

Antibody CAB075749

  
Immunocytochemistry


Validated
  

Validated
      
Immunohistochemistry


Validated
  

Validated
   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA032026

  

Antibody HPA032027

  

Antibody CAB032516

  

Antibody CAB075749

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA032027.		  
Validated

Antibody staining overlaps with antibody HPA032026.		      
 

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to vesicles.   Immunofluorescent staining of human cell line A-431 shows localization to peroxisomes.      

Antibody dilution

 1:78   1:35      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA032026

  

Antibody HPA032027

  

Antibody CAB032516

  

Antibody CAB075749

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 
     

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA032027 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA032026 across 46 cell lines.		      
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		      

 
     

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA032027 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA032026 across 73 cell types.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 41 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:75   1:50   1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA032026

  

Antibody HPA032027

  

Antibody CAB032516

  

Antibody CAB075749

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

        

Target mass (kDa)

 75.5   75.5   75.5, 27.1   75.5, 27.1  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA032026

  

Antibody HPA032027

  

Antibody CAB032516

  

Antibody CAB075749

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA032026

  

Antibody HPA032027

  

Antibody CAB032516

  

Antibody CAB075749

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Synthetic peptide  

Length (aa)

 65   55      

Antigen sequence

 
MVSQQEKGIEGVQVIPLIPGAGEIIIADNIIKFDHVPLATPNGDVLIRDL
NFEVRSGANVLICGP
  
VEGYIYSHCRKVGITLFTVSHRKSLWKHHEYYLHMDGRGNYEFKQITEDT
VEFGS
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ABCD3-001 - ENSP00000359233 [100%] 

											 
																						ABCD3-001 - ENSP00000359233 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ABCD3-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA032026

												
										 
																				
											
											
											
Antibody HPA032027

												
										 
																				
											
											
											
Antibody CAB032516

												
										 
																				
											
											
											
Antibody CAB075749

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Immunohistological analysis of ABCD3 expression in Caucasian and African American prostate tumors

	Reams RR et al

	Biomed Res Int 2015;2015:132981

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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