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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nuclear speckles & nucleoli fibrillar center.
Antibody dilution
1:56
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA034533
Antibody HPA064289
Standard validation
Approved
Figure description
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in a subset of bone marrow poietic cells.
Expression
RNA: detected in 34 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034533
Antibody HPA064289
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415541)
Target mass (kDa)
27.5, 26.4, 20.7, 20.5, 13.8
27.5, 26.4, 20.5, 12.7
Antibody dilution
1:250
1:190
PROTEIN ARRAY
Antibody HPA034533
Antibody HPA064289
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
