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KMT2A

ANTIBODY INFORMATION
 
 

Antibody HPA044910

  

Antibody CAB017794

  

Antibody CAB024270

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
   Upstate
  

Product name

 HPA044910   2530.00.02   05-765  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   msAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Protein A/G  

Other gene match

      

Released in version

 12   5   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA044910, RRID:AB_2679141      

VALIDATION SUMMARY
 
 

Antibody HPA044910

  

Antibody CAB017794

  

Antibody CAB024270

  
Immunocytochemistry

Supported      
Immunohistochemistry

  Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044910

  

Antibody CAB017794

  

Antibody CAB024270

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.      

Antibody dilution

 1:83      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044910

  

Antibody CAB017794

  

Antibody CAB024270

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:150   1:700  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044910

  

Antibody CAB017794

  

Antibody CAB024270

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

      

Target mass (kDa)

 432, 431.8, 155.5   432, 431.8, 155.5, 41.2, 28.4, 16.9, 8.6   432, 431.8, 155.5, 41.2, 28.4, 16.9, 8.6  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA044910

  

Antibody CAB017794

  

Antibody CAB024270

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:6000      

ANTIGEN INFORMATION
 
 

Antibody HPA044910

  

Antibody CAB017794

  

Antibody CAB024270

  

Antigen

 Recombinant protein fragment   Genetic immunization   Recombinant protein  

Length (aa)

 107      

Antigen sequence

 
DEQFLGFGSDEEVRVRSPTRSPSVKTSPRKPRGRPRSGSDRNSAILSDPS
VFSPLNKSETKSGDKIKKKDSKSIEKKRGRPPTFPGVKIKITHGKDISEL
PKGNKED
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					KMT2A-001 - ENSP00000436786 [100%] 
KMT2A-002 - ENSP00000432391 [100%] 
KMT2A-015 - ENSP00000374157 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
KMT2A-001
KMT2A-002
KMT2A-015
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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