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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in a subset of glial cells and neuropil was distinctly stained..
Expression
RNA: detected in 14 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034574
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
165.4, 61.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA034574
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.