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Antibody staining overlaps with antibody HPA062200.
Validated
Antibody staining overlaps with antibody HPA046917.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & mitochondria.
Antibody dilution
1:7
1:28
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA046917
Antibody HPA062200
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046917
Antibody HPA062200
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
242.4, 233.3
242.4, 233.3
Antibody dilution
1:80
1:90
PROTEIN ARRAY
Antibody HPA046917
Antibody HPA062200
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
