BCL11A - Antibodies - The Human Protein Atlas

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BCL11A

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA029003

Antibody CAB014891

Provider

Atlas Antibodies
Sigma-Aldrich

Origene

Product name

HPA029003

2125

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA029003, RRID:AB_2672863

VALIDATION SUMMARY

Antibody HPA029003

Antibody CAB014891

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA029003

Antibody CAB014891

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.

Antibody dilution

1:76

1:112

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA029003

Antibody CAB014891

Standard validation

Approved

Figure description

Immunohistochemical staining of human tonsil shows moderate nuclear positivity in leukocytes.

Immunohistochemical staining of human gall bladder shows cytoplasmic and nuclear positivity in glandular cells.

Expression

RNA: detected in 32 tissues
Protein: detected in 29 cell types

RNA: detected in 32 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA029003

Antibody CAB014891

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

91.2, 87.6, 83.9

91.2, 87.6, 83.9, 26.9, 21.1, 15.7, 2

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA029003

Antibody CAB014891

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA029003

Antibody CAB014891

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

Antigen sequence

LSTASSPEPGTSDLVGSASSALKSVVAKFKSENDPNLIPENGDEEEEEDD
EEEEEEEEEEEEELTESERVDYGFGLSL

Matching transcripts

BCL11A-001 - ENSP00000338774 [100%]
BCL11A-002 - ENSP00000349300 [100%]
BCL11A-006 - ENSP00000351307 [100%]

Other gene match

ANTIGEN VIEW

BCL11A-001	BCL11A-002	BCL11A-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.