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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

EPCAM

Gene description

Epithelial cell adhesion molecule

Protein class

Cancer-related genes
CD markers
Disease related genes
Plasma proteins
Predicted membrane proteins
Predicted secreted proteins

Predicted localization

Membrane,Secreted

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Mixed
HPA (cell line):Cell line enhanced (A-431, CACO-2, CAPAN-2, SCLC-21H)
HPA (normal tissue):Tissue enhanced (rectum, small intestine)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Selective expression in the cytoplasm and cell membranes of glandular cells, most abundant in the gastrointestinal tract and thyroid gland.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining consistent with RNA expression data.

Reliability score

Supported based on 4 antibodies.
HPA026761 , CAB003809 , CAB030012 , CAB055098
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (favourable) and ovarian cancer (favourable).
Renal cancer p<0.001
Ovarian cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Mixed

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Endometrial cancer

STAINING SUMMARY

HPA026761
CAB003809
CAB030012
CAB055098

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong membranous and cytoplasmic immunoreactivity was seen in most tumor tissues. Strongest immunoreactivity found in adenocarcinomas.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of malignant tissues displayed weak to moderate cytoplasmic and membranous immunoreactivity. Several colorectal cancers were strongly positive, while most malignant gliomas, lymphomas and melanomas were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Among malignant cells, moderate to strong membranous immunoreactivity was observed in a majority of cells. Malignant melanomas, lymphomas and gliomas were in general negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Colorectal, prostate, ovarian, endometrial, thyroid and lung cancers along with a few cases of breast and skin cancers exhibited strong membranous and cytoplasmic positivity. Remaining malignant tissues were negative.

GENE INFORMATION

Gene name

EPCAM (HGNC Symbol)

Synonyms

17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1

Description

Epithelial cell adhesion molecule (HGNC Symbol)

Entrez gene summary

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Chromosome

2

Cytoband

p21

Chromosome location (bp)

47345158 - 47387601

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000119888 (version 83.38)

Entrez gene

4072

UniProt

P16422 (UniProt - Evidence at protein level)

neXtProt

NX_P16422

Antibodypedia

EPCAM antibodies

PROTEIN BROWSER

EPCAM-001
EPCAM-002
EPCAM-006

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

EPCAM-001
ENSP00000263735
ENST00000263735
P16422 [Direct mapping]
Epithelial cell adhesion molecule
Show all
CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001657 [ureteric bud development]
GO:0005515 [protein binding]
GO:0005886 [plasma membrane]
GO:0005923 [bicellular tight junction]
GO:0008284 [positive regulation of cell proliferation]
GO:0009986 [cell surface]
GO:0016021 [integral component of membrane]
GO:0016323 [basolateral plasma membrane]
GO:0016324 [apical plasma membrane]
GO:0016328 [lateral plasma membrane]
GO:0023019 [signal transduction involved in regulation of gene expression]
GO:0032403 [protein complex binding]
GO:0043066 [negative regulation of apoptotic process]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0048863 [stem cell differentiation]
GO:0070062 [extracellular exosome]
GO:0098609 [cell-cell adhesion]
GO:0098641 [cadherin binding involved in cell-cell adhesion]
GO:2000048 [negative regulation of cell-cell adhesion mediated by cadherin]
GO:2000147 [positive regulation of cell motility]
GO:2000648 [positive regulation of stem cell proliferation]
Show all
314 aa
34.9 kDa
Yes 1
EPCAM-002
ENSP00000385476
ENST00000405271
B5MCA4 [Direct mapping]
Epithelial cell adhesion molecule
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0016021 [integral component of membrane]
Show all
342 aa
37.9 kDa
No 1
EPCAM-006
ENSP00000389028
ENST00000419334
C9JKY3 [Direct mapping]
Epithelial cell adhesion molecule
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
199 aa
21 kDa
Yes 0

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