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Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus & cell junctions.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027114
Antibody HPA027185
Standard validation
Supported
Figure description
Immunohistochemical staining of human fallopian tube shows strong cilia positivity in glandular cells.
Expression
RNA: detected in 21 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
Antibody dilution
1:1800
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027114
Antibody HPA027185
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410933)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410933)
Target mass (kDa)
82.3
82.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA027114
Antibody HPA027185
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
