HSPH1 - Antibodies - The Human Protein Atlas

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HSPH1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA028675

Antibody HPA031569

Antibody CAB002060

Provider

Atlas Antibodies
Sigma-Aldrich

Novocastra

Product name

HPA028675

HPA031569

NCL-HSP105

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA028675, RRID:AB_10601581

Atlas Antibodies Cat#HPA031569, RRID:AB_2673938

VALIDATION SUMMARY

Antibody HPA028675

Antibody HPA031569

Antibody CAB002060

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA028675

Antibody HPA031569

Antibody CAB002060

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Figure description

Whole cells of segmented cells in 10x-images

Antibody dilution

1:64

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA031569.

Validated

Antibody staining overlaps with antibody HPA028675.

Standard validation

Supported

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Antibody dilution

1:100

1:64

1:175

Literature conformity

The subcellular location is supported by literature.

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA028675

Antibody HPA031569

Antibody CAB002060

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in seminiferous ducts.

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 36 cell types

RNA: detected in 37 tissues
Protein: detected in 58 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

1:700

Literature conformity

Consistent with gene/protein characterization data.

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA028675

Antibody HPA031569

Antibody CAB002060

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

97.5, 96.9, 88.8

97.5, 96.9, 92.1, 88.8

97.5, 96.9, 92.1, 88.8, 13.2

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA028675

Antibody HPA031569

Antibody CAB002060

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA028675

Antibody HPA031569

Antibody CAB002060

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

TQPQVQTDAQQTSQSPPSPELTSEENKIPDADKANEKKVDQPPEAKKPKI
KVVNVELPIEANLVWQLG

IGRFVVQNVSAQKDGEKSRVKVKVRVNTHGIFTISTASMVEKVPTEENEM
SSEADMECLNQRPPENPDTDKNVQQDNSEAG

Matching transcripts

HSPH1-001 - ENSP00000318687 [100%]
HSPH1-006 - ENSP00000487365 [100%]
HSPH1-201 - ENSP00000396090 [100%]

HSPH1-001 - ENSP00000318687 [100%]
HSPH1-006 - ENSP00000487365 [100%]
HSPH1-201 - ENSP00000396090 [100%]
HSPH1-002 - ENSP00000369768 [88%]

Other gene match

ANTIGEN VIEW

HSPH1-001	HSPH1-002	HSPH1-006	HSPH1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.