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Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
Antibody dilution
1:96
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035945
Antibody HPA056766
Antibody CAB037212
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 54 cell types
RNA: detected in 37 tissues Protein: detected in 55 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:200
1:300
1:1500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035945
Antibody HPA056766
Antibody CAB037212
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413956)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
15
15, 11.4, 7.7
15, 11.4, 7.7
Antibody dilution
1:250
1:320
1:500
PROTEIN ARRAY
Antibody HPA035945
Antibody HPA056766
Antibody CAB037212
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.