Cell atlas - APAF1 - The Human Protein Atlas

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APAF1

TISSUE

CELL

PATHOLOGY

CELL ATLAS RNA EXPRESSION HUMAN CELLS

GENE/PROTEIN

Antibody validation

Dictionary
	Cytosol
	Golgi apparatus
	Nucleoplasm

Human cell
	Cytosol
	Golgi Apparatus
	Nucleoplasm

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GENERAL INFORMATION
Gene name	APAF1
Gene description	Apoptotic peptidase activating factor 1
Protein class	Cancer-related genes Plasma proteins Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	9
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nucleoplasm. In addition localized to the cytosol & the Golgi apparatus.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (approved)
Additional location	In addition localized to the Golgi apparatus (approved), Cytosol (approved)

DATA RELIABILITY
Reliability score	Approved based on 1 antibody. HPA031373
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nucleoplasm. In addition localized to the cytosol & the Golgi apparatus.

Main location

Nucleoplasm (approved)

Additional location

Golgi apparatus (approved), Cytosol (approved)


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Thumbnail	Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
	HPA031373	A-431	Nucleoplasm
			Golgi apparatus
			Cytosol
	HPA031373	U-2 OS	Nucleoplasm
	HPA031373	U-2 OS	Cytosol
	HPA031373	U-251 MG	Nucleoplasm
	HPA031373	U-251 MG	Cytosol

GENE INFORMATION
Gene name	APAF1 (HGNC Symbol)
Synonyms	APAF-1, CED4
Description	Apoptotic peptidase activating factor 1 (HGNC Symbol)
Entrez gene summary	This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Chromosome	12
Cytoband	q23.1
Chromosome location (bp)	98645141 - 98735433
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000120868 (version 83.38)
Entrez gene	317
UniProt	O14727 (UniProt - Evidence at protein level)
neXtProt	NX_O14727
Antibodypedia	APAF1 antibodies

PROTEIN BROWSER

APAF1-001	APAF1-002	APAF1-003	APAF1-004	APAF1-005	APAF1-006	APAF1-007	APAF1-201	APAF1-202

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
APAF1-001 ENSP00000353059 ENST00000359972	O14727 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mutational cancer driver genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005829 [cytosol] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0007399 [nervous system development] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0012501 [programmed cell death] GO:0042981 [regulation of apoptotic process] GO:0043065 [positive regulation of apoptotic process] GO:0043293 [apoptosome] GO:0043531 [ADP binding] GO:0070062 [extracellular exosome] GO:0072432 [response to G1 DNA damage checkpoint signaling] GO:0097193 [intrinsic apoptotic signaling pathway] Show all	1194 aa 136 kDa	No	0
APAF1-002 ENSP00000448165 ENST00000551964	O14727 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mutational cancer driver genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0001666 [response to hypoxia] GO:0001843 [neural tube closure] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005634 [nucleus] GO:0005829 [cytosol] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0007275 [multicellular organismal development] GO:0007399 [nervous system development] GO:0007420 [brain development] GO:0007568 [aging] GO:0007584 [response to nutrient] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0010659 [cardiac muscle cell apoptotic process] GO:0012501 [programmed cell death] GO:0030154 [cell differentiation] GO:0030900 [forebrain development] GO:0031072 [heat shock protein binding] GO:0034349 [glial cell apoptotic process] GO:0042802 [identical protein binding] GO:0042981 [regulation of apoptotic process] GO:0043065 [positive regulation of apoptotic process] GO:0043293 [apoptosome] GO:0043531 [ADP binding] GO:0051260 [protein homooligomerization] GO:0051402 [neuron apoptotic process] GO:0070059 [intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress] GO:0070062 [extracellular exosome] GO:0071560 [cellular response to transforming growth factor beta stimulus] GO:0072432 [response to G1 DNA damage checkpoint signaling] GO:0097190 [apoptotic signaling pathway] GO:0097193 [intrinsic apoptotic signaling pathway] GO:1902510 [regulation of apoptotic DNA fragmentation] GO:2001235 [positive regulation of apoptotic signaling pathway] Show all	1248 aa 141.8 kDa	No	0
APAF1-003 ENSP00000448826 ENST00000552268	O14727 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005829 [cytosol] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0007399 [nervous system development] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0012501 [programmed cell death] GO:0042981 [regulation of apoptotic process] GO:0043065 [positive regulation of apoptotic process] GO:0043293 [apoptosome] GO:0043531 [ADP binding] GO:0070062 [extracellular exosome] GO:0072432 [response to G1 DNA damage checkpoint signaling] GO:0097193 [intrinsic apoptotic signaling pathway] Show all	338 aa 38 kDa	No	0
APAF1-004 ENSP00000448449 ENST00000550527	O14727 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mutational cancer driver genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005829 [cytosol] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0007399 [nervous system development] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0012501 [programmed cell death] GO:0042981 [regulation of apoptotic process] GO:0043065 [positive regulation of apoptotic process] GO:0043293 [apoptosome] GO:0043531 [ADP binding] GO:0070062 [extracellular exosome] GO:0072432 [response to G1 DNA damage checkpoint signaling] GO:0097190 [apoptotic signaling pathway] GO:0097193 [intrinsic apoptotic signaling pathway] Show all	1237 aa 140.7 kDa	No	0
APAF1-005 ENSP00000449791 ENST00000547045	O14727 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005829 [cytosol] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0007399 [nervous system development] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0012501 [programmed cell death] GO:0042981 [regulation of apoptotic process] GO:0043065 [positive regulation of apoptotic process] GO:0043293 [apoptosome] GO:0043531 [ADP binding] GO:0070062 [extracellular exosome] GO:0072432 [response to G1 DNA damage checkpoint signaling] GO:0097190 [apoptotic signaling pathway] GO:0097193 [intrinsic apoptotic signaling pathway] Show all	1205 aa 137.1 kDa	No	0
APAF1-006 ENSP00000448161 ENST00000549007	C9JLV4 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0042981 [regulation of apoptotic process] GO:0043531 [ADP binding] Show all	1163 aa 132.5 kDa	No	0
APAF1-007 ENSP00000450175 ENST00000547743	F8VNZ0 [Direct mapping] Apoptotic protease-activating factor 1 Show all	SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0042981 [regulation of apoptotic process] Show all	130 aa 14.7 kDa	No	0
APAF1-201 ENSP00000334558 ENST00000333991	O14727 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mutational cancer driver genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005829 [cytosol] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0007399 [nervous system development] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0012501 [programmed cell death] GO:0042981 [regulation of apoptotic process] GO:0043065 [positive regulation of apoptotic process] GO:0043293 [apoptosome] GO:0043531 [ADP binding] GO:0070062 [extracellular exosome] GO:0072432 [response to G1 DNA damage checkpoint signaling] GO:0097193 [intrinsic apoptotic signaling pathway] Show all	338 aa 38 kDa	No	0
APAF1-202 ENSP00000349862 ENST00000357310	O14727 [Direct mapping] Apoptotic protease-activating factor 1 Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mutational cancer driver genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005829 [cytosol] GO:0006915 [apoptotic process] GO:0006919 [activation of cysteine-type endopeptidase activity involved in apoptotic process] GO:0007399 [nervous system development] GO:0008635 [activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c] GO:0008656 [cysteine-type endopeptidase activator activity involved in apoptotic process] GO:0012501 [programmed cell death] GO:0042981 [regulation of apoptotic process] GO:0043065 [positive regulation of apoptotic process] GO:0043293 [apoptosome] GO:0043531 [ADP binding] GO:0070062 [extracellular exosome] GO:0072432 [response to G1 DNA damage checkpoint signaling] GO:0097190 [apoptotic signaling pathway] GO:0097193 [intrinsic apoptotic signaling pathway] Show all	1205 aa 137.1 kDa	No	0

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The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.