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CD80

ANTIBODY INFORMATION
 
 

Antibody HPA050092

  

Antibody CAB025368

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA050092   MAB140  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 14   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA050092, RRID:AB_2681012    

VALIDATION SUMMARY
 
 

Antibody HPA050092

  

Antibody CAB025368

  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050092

  

Antibody CAB025368

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human tonsil shows distinct positivity in germinal and non-germinal center cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in inflammatory cells in stroma.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 18 cell types		   RNA: detected in 9 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050092

  

Antibody CAB025368

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 33, 29.4   33, 29.4  

Antibody dilution

 1:320   1:500  

PROTEIN ARRAY
 
 

Antibody HPA050092

  

Antibody CAB025368

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2600    

ANTIGEN INFORMATION
 
 

Antibody HPA050092

  

Antibody CAB025368

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 66    

Antigen sequence

 
YECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICS
TSGGFPEPHLSWLENG
  

											 
																				

																		

										
											
Matching transcripts

												
																					CD80-001 - ENSP00000264246 [100%] 
CD80-002 - ENSP00000418364 [100%] 
CD80-201 - ENSP00000373165 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

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CD80-001
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